Tissuelyser handbook 102010 5 a copy of qiagen terms and conditions can be obtained on request, and is also. Dna adsorbs to the dneasy membrane in the presence of high concentrations. To further evaluate the appropriateness of the qiaamp kit for ecological studies of gut microbiota, bacterial diversity in the digesta and mucosa of ilea and ceca were examined by pcrdgge analysis of dna samples prepared by the qiaamp kit and by bead beating. This product is not intended for the diagnosis, prevention, or treatment of a disease. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. Qiaamp dna mini kit and qiaamp dna blood mini kit handbook. All due care and attention should be exercised in the handling of the product. The benchguide protocol below only describes how to use the qiagen kit to purify dna that has already been extracted, and is thus a modified version of the original qiagen protocol. When using the qiaamp dna blood mini kit for a sample that requires a modified protocol. R20212232, s263646 buffer rlc contains guanidine hydrochloride. This protocol is designed for purification of total dna from grampositive bacteria.
Qiaamp viral rna mini handbook 122007 5 product use limitations the qiaamp viral rna mini kit is intended for general laboratory use. Qiaamp dna mini kit and qiaamp dna blood mini kit 022003 7 product use limitations qiaamp kits are intended as generalpurpose devices. For purification of up to 10 mg transfectiongrade plasmid or cosmid dna. Following the centrifugation, remove the dneasy mini spin column from the collection tube carefully so the column does not come into contact with the flowthrough, as this will result in carryover of ethanol. Qiagen provides a variety of qiaamp genomic dna kits for fast, efficient and easy dna. The protocol describes the preliminary harvesting of bacteria and incubation with lysozyme to lyse their cell walls before dna purification. Manual 12 automated 7 qiacube automated 4 biorobot automated 2. Qiaamp dna mini kit nucleic acid purification bacterial. Qiagen genomic tip kit algimed company, llc belarus.
Should any product fail to perform satisfactorily due to any reason other. Qiaamp mini spin columns can be easily processed in a centrifuge or on vacuum manifolds. The following risk and safety phrases apply to components of the rneasy mini kit, the rneasy protect mini kit, andor the rneasy plant mini kit. Using the qiaprep spin protocol, 10 g puc18 dna was purified and eluted with the indicated volumes of buffer eb. Purifying plasmid dna from bacterial colonies using the qiagen miniprep kit shenyuan zhang 1, michael d. Genomic dna contamination is effectively removed using a specially designed gdna eliminator spin column. The purified dna is free of proteins, nucleases and other contaminants and inhibitors. Plasmid or cosmid dna purification using qiagen plasmid mini kit 15 plasmid or cosmid dna purification using qiagen plasmid midi and maxi kits 19 plasmid or cosmid dna purification using qiagen plasmid mega and giga kits 24 very lowcopy plasmidcosmid purification using qiagen tip 100 or qiagen tip 500 29 troubleshooting guide 35. Follow the dna purification from blood or body fluids spin protocol procedure in the qiaamp dna mini kit handbook qiagen cat. Qiagen plasmid purification handbook harvard university. Rna collection 4 dna purification 1 viral dna and rna copurification 1 show all.
Please contact qiagen technical service at support. Qiaamp viral rna mini kit qiagen rna extraction protocol. Qiaamp dna mini kits enable rapid and efficient purification of highquality genomic, mitochondrial and viral dna from. Laboratory protocol for dna extraction from oracollectdx. All due care and attention should be exercised in the handling of the products. The purchaser must determine the suitability of the product for its particular use.
No claim or representation is intended for their use to identify any specific organism or for clinical use diagnostic, prognostic, therapeutic, or blood banking. The flexigene dna kit provides scalable purification of genomic dna. Rneasy mini handbook rneasy mini kit for purification of total rna from animal cells, animal tissues, bacteria, and yeast, and for rna cleanup rneasy protect mini kit for immediate stabilization of rna in harvested animal tissues and subsequent total rna purification rneasy plant mini kit for purification of total rna from plants and filamentous fungi fourth edition june. Com allprep dna rna mini handbook for simultaneous purification of genomic dna and total rna from the same animal cell or tissue sample.
Total rna and mirna can also be copurified from formalinfixed, paraffinembedded. For isolation of genomic, mitochondrial, bacterial, parasite or viral dna. The qiagen plasmid kits uses gravityflow qiagen anionexchange tips for efficient purification of plasmid dna. Qiaamp dna micro handbook 082003 7 introduction the qiaamp dna micro kit uses wellestablished technology for purification of genomic and mitochondrial dna from small sample volumes or sizes. Qiagen qiaamp dna mini kit, 50 preps, 200l sample, 50 to 200l elution volume, whole blood, tissue, cells sample, silica technology, spin column format, manual processing, 20 min. For purification of up to 12 g genomic, mitochondrial, or viral dna from blood and related body fluids. In comprehensive tests, the dneasy plant pro kit outperformed all other kits available, including the previous gold standard qiagen dneasy plant mini kit, isolating considerably more highquality dna for downstream next generation sequencing ngs, pcr and other applications. Dneasy plant mini kit and dneasy plant maxi kit handbook 082000 15 protocol protocol for isolation of dna from plant tissue with the dneasy plant mini kit important notes before starting if using dneasy plant mini kits for the first time please read technical information page 11. Dna purified using qiaamp kit is up to 50 kb in size, with fragments of approximately 2030 kb. Qiaamp dna mini and blood mini handbook moodle ufsc. The innovative qiacube uses advanced technology to process qiagen spin columns, enabling seamless integration of automated, lowthroughput sample prep into your laboratory workflow. The rnasefree dnase set is intended for molecular biology applications. Rna, proteins, dyes, and low molecular weight impurities are removed by a mediumsalt wash. Purelink genomic dna mini kit thermo fisher scientific.
Lyophilized qiagen protease can be stored at room temperature 1525c for up to 6 months without any decrease in performance. Genomic isolation is relatively straightforward and there are many kits available to do so. The quality of isolated dna is checked by restriction digestion. Extraction of dna from blood samples using qiaamp dna mini kit. For uptodate licensing information and productspecific disclaimers, see the respective qiagen kit handbook or user manual.
Allprep dna rnaprotein mini handbook 122014 5 productuselimitations the allprep dna rnaprotein mini kit is intended for molecular biology applications. Agarose gel electrophoresis analysis of the purifi ed samples showed that the integrity and quality of the dna was excellent. Mar 27, 2016 qiaamp viral rna mini kit qiagen rna extraction protocol. Qiaamp dna mini and blood mini handbook 112007 7 product use limitations the qiaamp dna mini kit and qiaamp dna blood mini kit are intended for general laboratory use. The qiaamp dna investigator kit provides purification of genomic dna from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. For the fastest, most convenient purification of up to 10 mg transfectiongrade plasmid dna.
Qiagen kit handbooks and user manuals are available at. Qiaamp dna blood mini kit 50 from qiagen selectscience. Qiagen plasmid kits are intended for molecular biology applications. Storage of rna later reagent at lower temperatures may cause precipitation. Qiagen protease is completely free of dnase and rnase activity. Laboratory protocol for dna extraction from oracollectdx ocd. When using the qiaamp dna blood mini kit for a sample that requires a. Qiaamp dna mini kit and qiaamp dna blood mini kit 092001 7 product use limitations qiaamp kits are intended as generalpurpose devices. The standard protocol uses 50 l buffer eb for elution, since this combines high yield with high concentration. Qiagen clinical kits compatible with the qiavac 24 plus application kit accessories genomic and viral dna purification from blood and body fluids qiaamp dna blood kits vacconnectors and vacvalves qiaamp dna mini kit vacconnectors and vacvalves viral rna purification from plasma, serum, and cellfree.
The qiaamp dna mini kit provides silicamembranebased nucleic acid purification from tissues, swabs, csf, blood, body fluids, or washed cells from urine. Timerun, 4 to 12g yield, rapid purification of highquality, readytouse dna, ideal for pcr, southern. The rneasy mini kit, rneasy protect mini kit including rna later rna stabilization reagent, and rneasy plant mini kit should be stored dry at room temperature 1525c and are stable for at least 9 months under these conditions. The qiaamp dna blood mini kit is designed for processing up to 200.
Hispeed plasmid purification handbook harvard university. Elution volume versus dna concentration and recovery. The dna yield of qiaprep miniprep kits is tested by preparing highcopy plasmid dna from 15 ml overnight culture in lb medium and assaying the dna yield spectrophotometrically. Purified dna is eluted from the dneasy mini spin column or dneasy 96 plate in. Qiaprep miniprep handbook washington state university. Cahalan 1 1 department of physiology and biophysics, university of california, irvine uci. Qiagen r dneasy r dna purification protocol mccouch. Qiaamp dna mini and blood mini handbook 052016 9 for purification of viral nucleic acids for in vitro diagnostics in europe, the qiaamp dsp virus kit is ceivdmarked, compliant with eu directive 9879ec. Jan 06, 2016 the general aim in using the qiaamp mini kit is to obtain reproducible results in our genomic dna preparations. The qiaamp dna blood mini kit provides silicamembranebased dna purification.
Dna mini kit on qiasymphony sp 196 samples per run. Dneasy powerplant pro kit handbook 052017 7 this kit provides powerbead tubes prefilled with 2. This offer is valid until december 31, 2019 and only when order is placed online through qiagen web shop, your organizations purchasing system, your sales rep, or by email using the cyber19 promo code. Our technical service departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of qiagen prod ucts. Alternative prefilled bead tube options are available for additional applications. Do not add bleach or acidic solutions directly to the samplepreparation waste. Dneasy plant handbook 102012 7 introduction dneasy plant kits provide a fast and easy way to purify dna from plant and fungal tissue. Automated dna cleanup the minelute pcr purification kit and minelute gel extraction kit can be fully automated on the qiacube. Qiagen qiaamp dna blood mini kit, 50 preps, 1 to 200l sample, 50 to 200l elution volume, whole blood, body fluids sample, spin column format, silica technology, manual processing, 20 to 40 min. Euresist qiaamp viral rna mini kit qiagen rna extraction. Our lab genotypes mice weekly and need a fast and reliable kit to obtain high quality genomic dna. For highthroughput purification in a 96well format, the mirneasy 96 kit is available. The qiagen dneasy kit includes instructions for grinding and extracting dna, as well as purifying it. These products are not intended for the diagnosis, prevention, or treatment of a disease.
The kit is compatible with a wide range of cultured cells and with easytolyse tissues. The qiaamp dna blood mini kit blood and body fluid spin protocol is used to provide a fast and easy method for purification of total genomic dna for reliable real time, quantitative pcr qpcr reactions. The convenient spincolumn procedure reduces handson preparation time to 20 minutes. No mechanical homogenization is necessary as the tissues are lysed enzymatically.
Dna is extracted from peripheral blood using qiaamp dna mini kit qiagen in accordance to the blood and body fluid protocol of the user. The kit combines the selective binding properties of a silicabased membrane with flexible elution volumes of. Evaluation of qiaamp dna stool mini kit for ecological. The kit uses qiaamp minelute spin columns for purification of highquality dna with flexible elution volumes. In doing so we can get our screening performed faster and save money in animal housing costs.
The qiaamp dna mini kit and qiaamp dna blood mini kit are intended for molecular biology applications. Up to 100 mg of tissue can be processed using the dneasy plant mini kit or up to 1 g of tissue using the dneasy plant maxi kit. Rneasy plus mini handbook 122014 7 introduction the rneasy plus mini kit is designed to purify rna from small amounts of animal cells or tissues. You can consult the dneasy plant handbook for more information. Purifying plasmid dna using the qiagen miniprep kit protocol. Qiaamp dna mini kit bacterial or genomic dna purification from water, filters, or surface swabs by qiagen. Qiagen plasmid purification handbook qiagen plasmid mini, midi, maxi, mega, and giga kits for purification of ultrapure, transfection grade plasmid dna third edition november 2005. These kits use the same silicamembrane technology as the qiaamp dna blood mini kit. Rneasy mini handbook 042006 7 the rneasy mini kit and rneasy protect mini kit are intended as generalpurpose devices. Refer to the handbook for protocols for swabs, dried blood spots, cultured cells, fixed tissue, bacteria, yeast or other material. We recommend all users of qiagen products to adhere to the nih guidelines. No claim or representation is intended for their use to identify any specific organism or for a specific clinical use diagnostic, prognostic, therapeutic, or blood banking. Qiaamp dna blood mini kit from qiagen biocompare product. Qiagen ipa powerful tools to uncover the significance of data and identify new targets or candidate biomarkers within the context of biological systems qiagen clc genomics workbench gain insights into your complex ngs data with our comprehensive toolkit for omics data analysis.
1060 198 62 1465 1209 429 306 908 277 1063 946 1640 667 951 1095 903 1483 164 619 823 951 583 1097 1002 1397 880 1145 451 1107 590 206 1438 297 1343 1141